Sequences database creation

Overview

Teaching: 20 min
Exercises: 20 min

Questions

• Why a curated DB is important?

• How can I create a DB?

• How can I prepare the DB for blast search?

Objectives

• Identify on NCBI the sequences I need

• Download them

• Prepare the sequences for blast search

Key Points

• There are other repositories other than NCBI

• Useful command of this lesson is: makeblastdb.

• The command takes options (flags) which begin with a -.